Goal-shooter Jhaniele Fowler-Reid came off the bench to lead Jamaica with 28 goals from 34 attempts. Romelda Aiken, who started in the shooting position, was 15 from 19. The England Roses top goal-shooter Jo Harten was 35 from 38, while goal-attack Pamela Cookey was 10 from 15.
The teams were tied 37-37 at the end of the third quarter, but the Sunshine Girls, riding on the energy of the vociferous home support, dug deep and put in a scintillating effort to stun third-ranked England.
The fourth-ranked hosts held a narrow 14-12 goal-lead at the end of the first quarter, but the scores were locked 24-24 at the half-time break.
The series was tied 1-1 after the teams shared the opening two games played on Saturday and Sunday.
Both countries are preparing for the Netball World Cup to be held in Australia in August.